LOCUS       sequence-83313-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J
  TITLE     Stable Enhanced Green Fluorescent Protein Expression After 
            Differentiation and Transplantation of Reporter Human Induced 
            Pluripotent Stem Cells Generated by AAVS1 Transcription 
            Activator-Like Effector Nucleases.
  JOURNAL   Stem Cells Transl Med. 2014 May 15. pii: sctm.2013-0212.
  PUBMED    24833591
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 May 15. pii: sctm.2013-0212."
COMMENT     Sequence Label: pZT-AAVS1-L1
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..611)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ctagactcga gttatcaaga tctaaagttt atctcgccgt tattaaattt ccgtctgact
       61 tcctctaagg ttaatgtgcc ggctttaatc atttctccac caattaaaag ctcttctaca
      121 ctaagaacag ctccattaca attagtgata tgatttaatc gtgtaagctg agctttgtag
      181 tttcctttaa agtgaccact cacaaataaa aacttaaatt ccgttacaga agatggatag
      241 actttccacc attcattagg gttgatatgt ttgtttcgtg tttgattttc ttcgacatat
      301 cgttgcattt catctgcttg gccaattggc agattataac ctccgctata agctttagta
      361 tccacgatca caccgtaatc aataggagat ccgacagtat aaattgctcc gtccggtttc
      421 cttgatccac ccaaatgttt acctctatat ccataaactt tcataaaaaa ttccattacc
      481 ttcatttcaa gaattctatc ctgagtggaa tttctggcaa tttcaattaa ttcaatatat
      541 tcatgaggca catatttcaa tttatgacga agttcagatt tcttctcctc cagttcactt
      601 ttgactagtt gggatcccgc gactcgatgg gaagttctct cgggaatccg ccggttggtt
      661 cttttgatca atgcgggagc atgaggcaga ccctttttga ctgcatcgag cgcgggacgt
      721 ccaccaagac atgccaacgc caccagatgg tcgttggtca acgcggccaa cgccggatca
      781 ggccggctca gctgggccac aatgctttcg agcgcttgct tgccgccacc gttgctggcg
      841 atagccacca cttggtccgg ggtcaggcca tggtcctggc acagcaccgg caacagccgc
      901 tgcaccgttt cgagcgcttg cttgccgcca ttgttgctgg cgatagccac cacttgg
//
