LOCUS       sequence-83315-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J
  TITLE     Stable Enhanced Green Fluorescent Protein Expression After 
            Differentiation and Transplantation of Reporter Human Induced 
            Pluripotent Stem Cells Generated by AAVS1 Transcription 
            Activator-Like Effector Nucleases.
  JOURNAL   Stem Cells Transl Med. 2014 May 15. pii: sctm.2013-0212.
  PUBMED    24833591
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 May 15. pii: sctm.2013-0212."
COMMENT     Sequence Label: pZT-AAVS1-R1
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..612)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tctagactcg agttatcaag atctaaagtt tatctcgccg ttattaaatt tccgtctgac
       61 ttcctctaag gttaatgtgc cggctttaat catttctcca ccaattaaaa gctcttctac
      121 actaagaaca gctccattac aattagtgat atgatttaat cgtgtaagct gagctttgta
      181 gtttccttta aagtgaccac tcacaaataa aaacttaaat tccgttacag aagatggata
      241 gactttccac cattcattag ggttgatatg tttgtttcgt gtttgatttt cttcgacata
      301 tcgttgcatt tcatctgctt ggccaattgg cagattataa cctccgctat aagctttagt
      361 atccacgatc acaccgtaat caataggaga tccgacagta taaattgctc cgtccggttt
      421 ccttgatcca cccaaatgtt tacctctata tccataaact ttcataaaaa attccattac
      481 cttcatttca agaattctat cctgagtgga atttctggca atttcaatta attcaatata
      541 ttcatgaggc acatatttca atttatgacg aagttcagat ttcttctcct ccagttcact
      601 tttgactagt tgggatcccg cgactcgatg ggaagttctc tcgggaatcc gccggttggt
      661 tcttttgatc aatgcgggag catgaggcag accctttttg actgcatcga gcgcgggacg
      721 tccaccaaga catgccaacg ccaccagatg gtcgttggtc aacgcggcca acgccggatc
      781 aggccggctc agctgggcca caatgctttc gagcgcttgc ttgccgccac cgttgctggc
      841 gatagccacc acttggtccg gggtcaggcc atggtcctgg cacagcaccg gcaacagccg
      901 ctgcaccgtt tcgagcgctt gcttgccgcc atcgtggctg gcgatagcca ccacttgg
//
