LOCUS       sequence-83331-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S and C699T)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             81..113
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(868..886)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(869..886)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(906..923)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt atggctagca tgactggtgg acagcaaatg ggtggatccg
      121 agtcctttgg gctgttctcc agcgtcatca atggggagga gcatgagcaa acccatcggg
      181 ctatattcag gtttgtgcct cggcatgaag atgaacttga gctggaagtg gatgaccccc
      241 tgctggtgga gctgcaggca gaagactatt ggtatgaggc ctataacatg cgcaccggag
      301 cccgcggggt cttccctgcc tactatgcca ttgaggtcac caaggagcct gagcacatgg
      361 cagcccttgc caaaaacagc gactggattg accagttccg ggtgaagttc ctggggtctg
      421 tccaggttcc ttatcacaag ggcaatgatg tcctctgtgc tgctatgcaa aagatcgcca
      481 ccacccgccg gctcaccgtg cactttaacc cgccctccag ctgtgtcctt gagatcagtg
      541 tcaggggtgt caagataggc gtcaaagctg atgatgctct ggaggccaag ggaaataaat
      601 gtagccactt cttccagcta aagaacatct ctttctgtgg ataccatcca aagaataaca
      661 agtactttgg gtttatcact aagcaccctg ctgaccaccg gtttgcctgc catgtctttg
      721 tgtctgaaga ttccaccaaa gccctggcgg agtctgtggg gcgtgcattt cagcagttct
      781 acaagcagtt tgtggagtat accactccta cagaagatat ctacttggag taggcggccg
      841 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
      901 atcagcctcg actgtgcctt ctagttgcca gccatc
//
