LOCUS       sequence-83339-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains expression cassette for NmCas9 with N and C 
            NLS and an HA tag, a cassette for expression of tracrRNA, a cassette
            for cloning crRNA under the control of U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..53
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttcaggtgt cgtgaacacc gccaccatgg tgcctaagaa gaagagaaag gtggctgcct
       61 tcaaacctaa ttcaatcaac tacatcctcg gcctcgatat cggcatcgca tccgtcggct
      121 gggcgatggt agaaattgac gaagaagaaa accccatccg cctgattgat ttgggcgtgc
      181 gcgtatttga gcgtgccgaa gtaccgaaaa caggcgactc ccttgccatg gcaaggcgtt
      241 tggcgcgcag tgttcgccgc ctgacccgcc gtcgcgccca ccgcctgctt cggacccgcc
      301 gcctattgaa acgcgaaggc gtattacaag ccgccaattt tgacgaaaac ggcttgatta
      361 aatccttacc gaatacacca tggcaacttc gcgcagccgc attagaccgc aaactgacgc
      421 ctttagagtg gtcggcagtc ttgttgcatt taatcaaaca tcgcggctat ttatcgcaac
      481 ggaaaaacga gggcgaaact gccgataagg agcttggcgc tttgcttaaa ggcgtagccg
      541 gcaatgccca tgccttacag acaggcgatt tccgcacacc ggccgaattg gctttaaata
      601 aatttgagaa agaaagcggc catatccgca atcagcgcag cgattattcg catacgttca
      661 gccgcaaaga tttacaggcg gagctgattt tgctgtttga aaaacaaaaa gaatttggca
      721 atccgcatgt ttcaggcggc cttaaagaag gtattgaaac cctactgatg acgcaacgcc
      781 ctgccctgtc cggcgatgcc gttcaaaaaa tgttggggca ttgcaccttc gaaccggcag
      841 agccgaaagc cgctaaaaac acctacacag ccgaacgttt catctggctg accaagctga
      901 acaacctgcg tattttagag caaggcagcg agcggccatt gaccgatacc gaacgcgcca
      961 cgcttatgga cgagccatac agaaaatcca aactgactta cgcacaagcc cgtaagctgc
     1021 tgggtttaga agataccgcc tttttcaaag gcttgcgcta tggtaaagac aatgccgaag
     1081 cctcaacatt gatggaaatg aaggcctacc atgccatcag ccgtgcactg gaaaa
//
