LOCUS       sequence-83340-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as 41024, with additional linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     various
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..223)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     144..168
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(274..363)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    join(589..607,608..630,631..649,650..672,673..691,692..714,
                     715..733,734..756,757..775,776..798,799..817,818..840,
                     841..859)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggatctatat taccctgtta tccctagcgg atctgccggt agaggtgtgg tcaataagag
       61 cgacctcata ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata
      121 agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt atttttttta
      181 taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta gaagtggcgc
      241 gccaccagca ccgtaatcag caaggcactg agcagcgtaa tctggaacgt catatggata
      301 ggatcctgca tagtccggga cgtcataggg atagcccgca tagtcaggaa catcgtatgg
      361 gtaaaagatg ttaattaaca tcctccagga tgataaacgg atcccccgaa ttgatccggt
      421 aatttagtgt gtgtatttgt gtttgcgtgt ctatagaagt atagtaattt atgctacaaa
      481 ggacctaatg tataaggaaa gaatatttag agaaaagaag aaaacaagag ttttatatac
      541 atacagagca catgcatgcc atagggtacc gagctcgact ttcacttttc tctatcactg
      601 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      661 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
      721 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
      781 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
      841 tctctatcac tgatagggag tggtaaactc gatcgaggaa ttgatctata ttacc
//
