LOCUS       sequence-83343-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains expression cassette for NmCas9 with N and C 
            NLS and an HA tag, a cassette for expression of tracrRNA, a cassette
            for cloning crRNA under the control of U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..59)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tccagcttct ttttcttcgc tgctggagct gcagcgtaat ctggaacatc gtatgggtaa
       61 cggacaggcg ggcgtttttt cagacggcat ggtctgattt ctttgcccag ttcgtcaatt
      121 tggtattttt ggaatgaaag ggcggttttg acgccgatac cttccagtat tccatttttg
      181 ccaattttat gatcaagatc atgaatgcgt atattgatat tacctgtgcc tcgatggcag
      241 ctggcaaagt aaccaaacat tctagctttt tttgttataa cctcgactaa atcattaggg
      301 tgtaatgaga atttaaagtt gaaactatca tcaataagtt gccaatcttc ttcatctttt
      361 ccttgtacaa cagccctatc cggcaaaatc cctttcgcta cctgccaact gtaaatcggt
      421 accagataat acttgtcgcc tttctcaaac acatctacgc gcaccatggt tgcgttgtcg
      481 gcaataccgt tatggttgcg cacccatacg ccggttttct gtacttgctc tacgcgtacg
      541 gcttttacct gttgggtgcg gttgcctgct ttatcgtatt tgtaaaacgg ctcggcaaag
      601 gctttggcag gatcgtcttt atgtgcttcc agccgtgctt tcagtgcttc gtatagctta
      661 ggttcgcgct cccgattgac cattttttcc aagtctttca gttttaactg tgtcagcggt
      721 acgcgcaaca cgctgacgcc ttcgtccagt cgtttggcgg atttgacggt ctccatatgc
      781 ccttgcccgc tcatcttccg attgggcgca cgtgaaacaa acagtggcgt aacgtattcg
      841 tgtacggctt cggggcgaga tgataatttt tcggcaagca acgtgcgcag tttttctagg
      901 gtatcggctt cttcgaattc gggtttgccg tccggtttgc cgaagacgcg aatcatgact
      961 tcttgtgcga aaaattccca aggttgtggg aagtgtgttt tttgatgcag cacttctcct
     1021 gtttctttgt ctatggtttt accgtcaaac gcgttcatct ctttatagcg tacaaaacgg
     1081 gtaattttct gctgcatggc aacggtcgag caggcaacga cgacgg
//
