LOCUS       sequence-83351-e        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AavLEA1 expression (in BL21 DE3 E. coli strain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Tripathi R, Benz N, Culleton B, Trouve P, Ferec C
  TITLE     Biophysical Characterisation of Calumenin as a Charged F508del-CFTR 
            Folding Modulator.
  JOURNAL   PLoS One. 2014 Aug 13;9(8):e104970. doi: 
            10.1371/journal.pone.0104970. eCollection 2014.
  PUBMED    25120007
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-08-13"; volume: "9(8):e104970. doi"; pages: " 
            10.1371/journal.pone.0104970. eCollection 2014"
COMMENT     Sequence Label: pET15b-AavLEA1
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             166..183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             193..210
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(696..714)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      710..757
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(932..937,938..954,955..960))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(987..1005)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
      181 cacagcagcg gcctggtgcc gcgcggcagc catatgtcct ctcagcagaa ccagaaccga
      241 cagggtgagc agcaggagca gggctacatg gaggcggcca aggagaaggt cgtcaacgca
      301 tgggagagca cgaaggaaac cctctcgagc acggctcaag cggccgccga gaagacggct
      361 gagtttcgcg attccgccgg tgagaccatc cgtgacctga ccggacaggc gcaggagaag
      421 ggtcaggagt tcaaggagcg cgctggcgag aaggcagagg agacgaagca gcgtgccggg
      481 gagaagatgg atgagaccaa gcagcgggct ggcgaaatgc gcgagaacgc gggccagaag
      541 atggaggagt acaagcagca gggcaagggc aaggccgagg agcttcgcga cactgccgcc
      601 gagaagctcc accaggctgg cgagaaggtc aagggccgcg actaaggatc cggctgctaa
      661 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      721 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      781 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
      841 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
      901 cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa
      961 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1021 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1081 ccctatttgt ttatttttct aaatacattc aaatatgt
//
