LOCUS       sequence-83352-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter (luciferase) for interferon signalling in fish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Collet B, Boudinot P, Benmansour A, Secombes CJ
  TITLE     An Mx1 promoter-reporter system to study interferon pathways in 
            rainbow trout.
  JOURNAL   Dev Comp Immunol. 2004 Jun . 28(7-8):793-801.
  PUBMED    15043947
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Comp 
            Immunol. 2004 Jun . 28(7-8):793-801."
COMMENT     Sequence Label: pGL3-pomMx1
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..882
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             88..882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(142..161)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     752..771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
       61 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
      121 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      181 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      241 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
      301 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      361 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      421 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      481 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
      541 cttgtcgatc aggatgatct ggacgaagaa catcaggggc tcgcgccagc cgaactgttc
      601 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
      661 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
      721 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagaa
      781 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
      841 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
      901 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
      961 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     1021 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacac
//
