LOCUS       sequence-83365-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcDNA3 T7 Jip1b(471-707)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(766..798)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(875..926)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(904..924)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc ctatccaagt agatatcttc
       61 tgtaggacag gtatactcca caaactgctt gtagaactgc tgaaatgcac gccccacaga
      121 ctccgccagg gctttggtgg aatcttcaga cacaaagaca tggcaggcaa accggtggtc
      181 agcagggtgc ttagtgataa acccaaagta cttgttattc tttggatggt atccacagaa
      241 agagatgttc tttagctgga agaagtggct acatttattt cccttggcct ccagagcatc
      301 atcagctttg acgcctatct tgacacccct gacactgatc tcaaggacac agctggaggg
      361 cgggttaaag tgcacggtga gccggcgggt ggtggcgatc ttttgcatag cagcacagag
      421 gacatcattg cccttgtgat aaggaacctg gacagacccc aggaacttca cccggaactg
      481 gtcaatccag tcgctgtttt tggcaagggc tgccatgtgc tcaggctcct tggtgacctc
      541 aatggcatag taggcaggga agaccccgcg ggctccggtg cgcatgttat aggcctcata
      601 ccaatagtct tctgcctgca gctccaccag cagggggtca tccacttcca gctcaagttc
      661 atcttcatgc cgaggcacaa acctgaatat agcccgatgg gtttgctcat gctcctcccc
      721 attgatgacg caggagaaca gcccaaagga ctctcctccg gatccaccca tttgctgtcc
      781 accagtcatg ctagccataa gcttgggtct ccctatagtg agtcgtatta atttcgataa
      841 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      901 gtacacgcct accgcccatt tgcgtcaatg
//
