LOCUS       sequence-8338-ex        6630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
  TITLE     Optimized large-scale production of high titer lentivirus vector 
            pseudotypes.
  JOURNAL   J Virol Methods. 2004 Dec 15. 122(2):131-9.
  PUBMED    15542136
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol 
            Methods. 2004 Dec 15. 122(2):131-9."
COMMENT     Sequence Label: pHCMV-RabiesG
FEATURES             Location/Qualifiers
     source          1..6630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3334..3353)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3399..3454
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3453..3472)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3812..3828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3812..3828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3825..3847)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3836..3852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3860..3866,3867..3884,3885..3890))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3905..3926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4043..4060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4214..4802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4294..4313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5271..5650)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     5597..5616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5835..5939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5965..6420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6102..6123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6314..6333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6547..6569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6562..6578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6588..6607
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6588..6606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatct gtcgacggta
     1441 tcgataagct tgatatcgaa ttcggcacga gggaaagatg gttcctcagg ctctcctgtt
     1501 tgtacccctt ctggtttttc cattgtgttt tgggaaattc cctatttaca cgataccaga
     1561 caagcttggt ccctggagtc cgattgacat acatcacctc agctgcccaa acaatttggt
     1621 agtggaggac gaaggatgca ccaacctgtc agggttctcc tacatggaac ttaaagttgg
     1681 atacatctta gccataaaag tgaacgggtt cacttgcaca ggcgttgtga cggaggctga
     1741 aacctacact aacttcgttg gttatgtcac aaccacgttc aaaagaaagc atttccgccc
     1801 aacaccagat gcatgtagag ccgcgtacaa ctggaagatg gccggtgacc ccagatatga
     1861 agagtctcta cacaatccgt accctgacta ccgctggctt cgaactgtaa aaaccaccaa
     1921 ggagtctctc gttatcatat ctccaagtgt ggcagatttg gacccatatg acagatccct
     1981 tcactcgagg gtcttcccta gcgggaagtg ctcaggagta gcggtgtctt ctacctactg
     2041 ctccactaac cacgattaca ccatttggat gcccgagaat ccgagactag ggatgtcttg
     2101 tgacattttt accaatagta gagggaagag agcatccaaa gggagtgaga cttgcggctt
     2161 tgtagatgaa agaggcctat ataagtcttt aaaaggagca tgcaaactca agttatgtgg
     2221 agttctagga cttagactta tggatggaac atgggtctcg atgcaaacat caaatgaaac
     2281 caaatggtgc cctcccgata agttggtgaa cctgcacgac tttcgctcag acgaaattga
     2341 gcaccttgtt gtagaggagt tggtcaggaa gagagaggag tgtctggatg cactagagtc
     2401 catcatgaca accaagtcag tgagtttcag acgtctcagt catttaagaa aacttgtccc
     2461 tgggtttgga aaagcatata ccatattcaa caagaccttg atggaagccg atgctcacta
     2521 caagtcagtc agaacttgga atgagatcct cccttcaaaa gggtgtttaa gagttggggg
     2581 gaggtgtcat cctcatgtga acggggtgtt tttcaatggt ataatattag gacctgacgg
     2641 caatgtctta atcccagaga tgcaatcatc cctcctccag caacatatgg agttgttgga
     2701 atcctcggtt atcccccttg tgcaccccct ggcagacccg tctaccgttt tcaaggacgg
     2761 tgacgaggct gaggattttg ttgaagttca ccttcccgat gtgcacaatc aggtctcagg
     2821 agttgacttg ggtctcccga actgggggaa gtatgtatta ctgagtgcag gggccctgac
     2881 tgccttgatg ttgataattt tcctgatgac atgttgtaga agagtcaatc gatcagaacc
     2941 tacgcaacac aatctcagag ggacagggag ggaggtgtca gtcactcccc aaagcgggaa
     3001 gatcatatct tcatgggaat cacacaagag tgggggtgag accagactgt aaggactggc
     3061 cgtcctttca acgatccaag tcctgaagat cacctcccct tggggggttc tttttgaaaa
     3121 acctgggttc aatagtcctc cttgaactcc atgcaactgg gtagattcaa gagtcatgag
     3181 attttcatta atcctctcag ttgatcaagc aagatcatgt cgattctcat aataggggag
     3241 atccgtcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     3301 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     3361 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     3421 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     3481 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     3541 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     3601 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     3661 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     3721 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     3781 cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     3841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4621 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcgggttagc
     5401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5581 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5941 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     6001 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6061 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6121 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6181 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6241 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6301 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6361 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6421 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6481 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6541 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     6601 tatagggcga attggagctc caccgcggtg
//
