LOCUS       sequence-83392-e        4590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Hartzog PE, Nicholson BP, McCusker JH
  TITLE     Cytosine deaminase MX cassettes as positive/negative selectable 
            markers in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2005 Jul 30;22(10):789-98.
  PUBMED    16088873
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2005-07-30"; volume: "22(10)"; pages: "789-98"
COMMENT     Sequence Label: pPH 34
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        581..924
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1453..1650
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2227..2246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2228..2246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2333..2350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2504..3092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2584..2603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3263..4054,4055..4123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3886..3905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4124..4228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4296..4314
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4352..4374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4474..4493
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4574..4590,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4574..4590,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctgatct gcccgccggg aaggcgaacc cgatcggatg catcctctct
      121 gctgccatga tgctgaagtt gtcgttgaac atggttgctg ccggcgaggc ggtcgagcag
      181 gcagtgcagg aggtgttgga ctcgggagtc agaacgggcg acctgctcgg ctcgagctcc
      241 acttcggagg ttggcgacgc cattgcgctt gcagttaagg aagccttgcg caggcaatcc
      301 gcagctggtc tgagctagcc tcgaggaccc ttctctttag actattctac tcttatgcac
      361 gtaaaaaatt ctaggaaata tgtattaact aggagtaaaa taaccggcta gtggcattca
      421 tatagccgtc tgtttacatc tacatcacac atttcgagtg tatatctcgc aacgttggcg
      481 ttaaataggc agtcaatggc ccgaccattc tatggtgttt aggtcgatgc catctttgta
      541 cgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
      601 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      661 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      721 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      781 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      841 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      901 catcacatcc gaacataaac aaccatgacg tttgacgaca aaaaaggttt acaagttgct
      961 cttgatcaag ccaagaaaag tatgtgtata tcatgcccac tctgaacttt caattaaaat
     1021 ccagctttac taacacactt gctgtccagg ttactcagaa ggtgggatac ctattggttc
     1081 atgtattatt tcatctgatg acacagtatt aggccaaggt cacaacgaaa gaatccaaaa
     1141 acattcagct attttacatg gagaaatgtc agcattagaa aacgcaggaa gattaccagg
     1201 aaaaacttat aaagattgta ccatctatac tactttatca ccatgtagta tgtgtactgg
     1261 ggccatttta ttatatggat tcaaaagagt tgtgatggga gaaaatgtca atttcttggg
     1321 taatgaaaag ttattgattg aaaatggtgt cgaagttgtg aatttaaatg atcaagaatg
     1381 tattgatttg atggccaaat tcattaaaga gaaaccacaa gattggaatg aagatattgg
     1441 agaataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1501 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1561 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1621 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1681 gtgtcgagat ctgcccgccg ggaaggcgaa cccgatcgga tgcatcctct ctgctgccat
     1741 gatgctgaag ttgtcgttga acatggttgc tgccggcgag gcggtcgagc aggcagtgca
     1801 ggaggtgttg gactcgggag tcagaacggg cgacctgctc ggctcgagct ccacttcgga
     1861 ggttggcgac gccattgcgc ttgcagttaa ggaagccttg cgcaggcaat ccgcagctgg
     1921 tctgagctag cctcgaggac ccttctcttt agactattct actcttatgc acgtaaaaaa
     1981 ttctaggaaa tatgtattaa ctaggagtaa aataaccggc tagtggcatt catatagccg
     2041 tctgtttaca tctacatcac acatttcgag tgtatatctc gcaacgttgg cgttaaatag
     2101 gcagtcaatg gcccgaccat tctatggtgt ttaggtcgat gccatctttg tacgtttaaa
     2161 cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc gcggatctgc
     2221 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
     2281 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2341 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2401 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2461 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2521 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2581 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2641 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     2701 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2761 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2821 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2881 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2941 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3001 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3061 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4261 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4321 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4381 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4441 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4501 tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
     4561 tcatacacat acgatttagg tgacactata
//
