LOCUS       sequence-83397-e        4512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Hartzog PE, Nicholson BP, McCusker JH
  TITLE     Cytosine deaminase MX cassettes as positive/negative selectable 
            markers in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2005 Jul 30;22(10):789-98.
  PUBMED    16088873
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2005-07-30"; volume: "22(10)"; pages: "789-98"
COMMENT     Sequence Label: pBN63
FEATURES             Location/Qualifiers
     source          1..4512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        565..908
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1391..1588
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2149..2168)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2150..2168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2255..2272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2426..3014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2506..2525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3185..3976,3977..4045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3808..3827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4046..4150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4218..4236
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4274..4296)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4396..4415
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4496..4512,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4496..4512,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctcatta ttgcactggg tgttctgggc gggactgcac acgctgccaa
      121 tgaagtaact ttcctgggtt cagtaagcgc aacaacctgt gacctgacca cttctgtcaa
      181 cggcgccgct cagccgaacc aggttgttca gctgggtacc gttcaggcta accagccggg
      241 taattttgtt gacttcgcca tgaaaccggt tgacccgaat gcacagggtt gtgcaaatct
      301 ggctcagaaa acggccactg tttcctgggc ttctgctgcc ctggatggcg aaggctttgg
      361 ggcaaccagc ggtacagcta ctgatgccaa agtactggtt gaaagcgtga actccaaaaa
      421 cccgggggct gtaaacgcta acgccagcac ggttgacttt gaaggtgcca agctgaccac
      481 cgacggcctg cagttcaaag ccaagctgaa aggtggtgcg accgagttta gcttgcctcg
      541 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
      601 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
      661 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
      721 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
      781 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
      841 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
      901 aaacaaccat ggtgacaggg ggaatggcaa gcaagtggga tcagaagggt atggacattg
      961 cctatgagga ggcggcctta ggttacaaag agggtggtgt tcctattggc ggatgtctta
     1021 tcaataacaa agacggaagt gttctcggtc gtggtcacaa catgagattt caaaagggat
     1081 ccgccacact acatggtgag atctccactt tggaaaactg tgggagatta gagggcaaag
     1141 tgtacaaaga taccactttg tatacgacgc tgtctccatg cgacatgtgt acaggtgcca
     1201 tcatcatgta tggtattcca cgctgtgttg tcggtgagaa cgttaatttc aaaagtaagg
     1261 gcgagaaata tttacaaact agaggtcacg aggttgttgt tgttgacgat gagaggtgta
     1321 aaaagatcat gaaacaattt atcgatgaaa gacctcagga ttggtttgaa gatattggtg
     1381 agtagtcagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     1441 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     1501 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     1561 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt
     1621 gtcgacatta ttgcactggg tgttctgggc gggactgcac acgctgccaa tgaagtaact
     1681 ttcctgggtt cagtaagcgc aacaacctgt gacctgacca cttctgtcaa cggcgccgct
     1741 cagccgaacc aggttgttca gctgggtacc gttcaggcta accagccggg taattttgtt
     1801 gacttcgcca tgaaaccggt tgacccgaat gcacagggtt gtgcaaatct ggctcagaaa
     1861 acggccactg tttcctgggc ttctgctgcc ctggatggcg aaggctttgg ggcaaccagc
     1921 ggtacagcta ctgatgccaa agtactggtt gaaagcgtga actccaaaaa cccgggggct
     1981 gtaaacgcta acgccagcac ggttgacttt gaaggtgcca agctgaccac cgacggcctg
     2041 cagttcaaag ccaagctgaa aggtggtgcg accgagttta aacgagctcg aattcatcga
     2101 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
     2161 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
     2221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     2641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3061 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3301 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4141 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4201 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4261 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4321 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4381 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat
     4441 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     4501 ggtgacacta ta
//
