LOCUS       sequence-83398-e        4558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Hartzog PE, Nicholson BP, McCusker JH
  TITLE     Cytosine deaminase MX cassettes as positive/negative selectable 
            markers in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2005 Jul 30;22(10):789-98.
  PUBMED    16088873
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2005-07-30"; volume: "22(10)"; pages: "789-98"
COMMENT     Sequence Label: pBN62
FEATURES             Location/Qualifiers
     source          1..4558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        565..908
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1437..1634
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2195..2214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2196..2214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2301..2318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2472..3060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2552..2571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3231..4022,4023..4091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3854..3873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4092..4196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4264..4282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4320..4342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4442..4461
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4542..4558,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4542..4558,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctcatta ttgcactggg tgttctgggc gggactgcac acgctgccaa
      121 tgaagtaact ttcctgggtt cagtaagcgc aacaacctgt gacctgacca cttctgtcaa
      181 cggcgccgct cagccgaacc aggttgttca gctgggtacc gttcaggcta accagccggg
      241 taattttgtt gacttcgcca tgaaaccggt tgacccgaat gcacagggtt gtgcaaatct
      301 ggctcagaaa acggccactg tttcctgggc ttctgctgcc ctggatggcg aaggctttgg
      361 ggcaaccagc ggtacagcta ctgatgccaa agtactggtt gaaagcgtga actccaaaaa
      421 cccgggggct gtaaacgcta acgccagcac ggttgacttt gaaggtgcca agctgaccac
      481 cgacggcctg cagttcaaag ccaagctgaa aggtggtgcg accgagttta gcttgcctcg
      541 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
      601 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
      661 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
      721 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
      781 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
      841 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
      901 aaacaaccat gacgtttgac gacaaaaaag gtttacaagt tgctcttgat caagccaaga
      961 aaagtatgtg tatatcatgc ccactctgaa ctttcaatta aaatccagct ttactaacac
     1021 acttgctgtc caggttactc agaaggtggg atacctattg gttcatgtat tatttcatct
     1081 gatgacacag tattaggcca aggtcacaac gaaagaatcc aaaaacattc agctatttta
     1141 catggagaaa tgtcagcatt agaaaacgca ggaagattac caggaaaaac ttataaagat
     1201 tgtaccatct atactacttt atcaccatgt agtatgtgta ctggggccat tttattatat
     1261 ggattcaaaa gagttgtgat gggagaaaat gtcaatttct tgggtaatga aaagttattg
     1321 attgaaaatg gtgtcgaagt tgtgaattta aatgatcaag aatgtattga tttgatggcc
     1381 aaattcatta aagagaaacc acaagattgg aatgaagata ttggagaata atcagtactg
     1441 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     1501 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     1561 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     1621 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg acattattgc
     1681 actgggtgtt ctgggcggga ctgcacacgc tgccaatgaa gtaactttcc tgggttcagt
     1741 aagcgcaaca acctgtgacc tgaccacttc tgtcaacggc gccgctcagc cgaaccaggt
     1801 tgttcagctg ggtaccgttc aggctaacca gccgggtaat tttgttgact tcgccatgaa
     1861 accggttgac ccgaatgcac agggttgtgc aaatctggct cagaaaacgg ccactgtttc
     1921 ctgggcttct gctgccctgg atggcgaagg ctttggggca accagcggta cagctactga
     1981 tgccaaagta ctggttgaaa gcgtgaactc caaaaacccg ggggctgtaa acgctaacgc
     2041 cagcacggtt gactttgaag gtgccaagct gaccaccgac ggcctgcagt tcaaagccaa
     2101 gctgaaaggt ggtgcgaccg agtttaaacg agctcgaatt catcgatgat atcagatcca
     2161 ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg
     2221 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2281 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2641 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2881 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3121 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3181 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3541 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3841 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4201 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4261 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4321 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4381 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4441 gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc
     4501 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactata
//
