LOCUS       sequence-83399-e        4789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Hartzog PE, Nicholson BP, McCusker JH
  TITLE     Cytosine deaminase MX cassettes as positive/negative selectable 
            markers in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2005 Jul 30;22(10):789-98.
  PUBMED    16088873
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2005-07-30"; volume: "22(10)"; pages: "789-98"
COMMENT     Sequence Label: pBN100
FEATURES             Location/Qualifiers
     source          1..4789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        565..908
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1727..1924
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2485..2504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2486..2504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2591..2608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2762..3350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2842..2861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3521..4312,4313..4381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4144..4163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4382..4486)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4554..4572
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4610..4632)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4732..4751
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctcatta ttgcactggg tgttctgggc gggactgcac acgctgccaa
      121 tgaagtaact ttcctgggtt cagtaagcgc aacaacctgt gacctgacca cttctgtcaa
      181 cggcgccgct cagccgaacc aggttgttca gctgggtacc gttcaggcta accagccggg
      241 taattttgtt gacttcgcca tgaaaccggt tgacccgaat gcacagggtt gtgcaaatct
      301 ggctcagaaa acggccactg tttcctgggc ttctgctgcc ctggatggcg aaggctttgg
      361 ggcaaccagc ggtacagcta ctgatgccaa agtactggtt gaaagcgtga actccaaaaa
      421 cccgggggct gtaaacgcta acgccagcac ggttgacttt gaaggtgcca agctgaccac
      481 cgacggcctg cagttcaaag ccaagctgaa aggtggtgcg accgagttta gcttgcctcg
      541 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
      601 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
      661 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
      721 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
      781 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
      841 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
      901 aaacaaccat gacagtcaac actaagacct atagtgagag agcagaaact catgcctcac
      961 cagtagcaca gcgattattt cgattaatgg aactgaagaa aaccaattta tgtgcatcaa
     1021 ttgacgttga taccactaag gaattccttg aattaattga taaattaggt ccttatgtat
     1081 gcttaatcaa gactcatatt gatataatca atgatttttc ctatgaatcc actattgaac
     1141 cattattaga actttcacgt aaacatcaat ttatgatttt tgaagataga aaatttgctg
     1201 atattggtaa taccgtaaag aaacaatata ttggtggagt ttataaaatt agtagttggg
     1261 cagatattac caatgctcat ggtgtcactg ggaatggagt ggttgaagga ttaaaacagg
     1321 gagctaaaga aaccaccacc aaccaagagc caagagggtt attgatgtta gctgaattat
     1381 catcagtggg atcattagca tatggagaat attctcaaaa aactgttgaa attgctaaat
     1441 ccgataagga atttgttatt ggatttattg cccaacgtga tatgggtggc caagaagaag
     1501 gatttgattg gcttattatg acacctggag ttggattaga tgataaaggt gatggattag
     1561 gacaacaata tagaactgtt gatgaagttg ttagcactgg aactgatatt atcattgttg
     1621 gtagaggatt gtttggtaaa ggaagagatc cagatattga aggtaaaagg tatagaaatg
     1681 ctggttggaa tgcttatttg aaaaagactg gccaattata atcagtactg acaataaaaa
     1741 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     1801 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     1861 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     1921 actgctgtcg attcgatact aacgccgcca tccagtgtcg acattattgc actgggtgtt
     1981 ctgggcggga ctgcacacgc tgccaatgaa gtaactttcc tgggttcagt aagcgcaaca
     2041 acctgtgacc tgaccacttc tgtcaacggc gccgctcagc cgaaccaggt tgttcagctg
     2101 ggtaccgttc aggctaacca gccgggtaat tttgttgact tcgccatgaa accggttgac
     2161 ccgaatgcac agggttgtgc aaatctggct cagaaaacgg ccactgtttc ctgggcttct
     2221 gctgccctgg atggcgaagg ctttggggca accagcggta cagctactga tgccaaagta
     2281 ctggttgaaa gcgtgaactc caaaaacccg ggggctgtaa acgctaacgc cagcacggtt
     2341 gactttgaag gtgccaagct gaccaccgac ggcctgcagt tcaaagccaa gctgaaaggt
     2401 ggtgcgaccg agtttaaacg agctcgaatt catcgatgat atcagatcca ctagtggcct
     2461 atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg
     2521 ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2941 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3181 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4501 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4561 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4621 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4681 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     4741 agcagattgt actgagagtg caccatatgg acatattgtc gttagaacg
//
