LOCUS       sequence-83409-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The expression vector for humanized nickase Cas9 (Cas9D10A) under 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     pCAG-hCas9D10A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-hCas9D10A
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(138..158)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcaataaaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gggggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      121 agagtcgcgg ccgctcacac cttcctcttc ttcttggggt cagccctgct gtctccaccg
      181 agctgagaga ggtcgattct tgtttcatag agccccgtaa ttgactgatg aatcagtgtg
      241 gcgtccagga cctcctttgt agaggtgtac cgctttctgt ctatggtggt gtcgaagtac
      301 ttgaaggctg caggcgcgcc caagttggtc agagtaaaca agtggataat gttttctgcc
      361 tgctccctga tgggcttatc cctgtgctta ttgtaagcag aaagcacctt atcgaggtta
      421 gcgtcggcga ggatcactct tttggagaat tcgcttattt gctcgatgat ctcatcaagg
      481 tagtgtttgt gttgttccac gaacagctgc ttctgctcat tatcttcggg agaccctttg
      541 agcttttcat agtggctggc cagatacaag aaattaacgt atttagaggg cagtgccagc
      601 tcgttacctt tctgcagctc gcccgcacta gcgagcattc gtttccggcc gttttcaagc
      661 tcaaagagag agtacttggg aagcttaatg atgaggtctt ttttgacctc tttatatcct
      721 ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg atcgctccat gattgtgatg
      781 cccagcagtt ccttgacgct tttgagtttt ttagacttcc ctttctccac tttggccaca
      841 accagtacac tgtaagcgac tgtaggagaa tcgaatccgc cgtatttctt ggggtcccaa
      901 tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg ggaggatact tt
//
