LOCUS       sequence-83423-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: LSL K-ras G12D
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        149..253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             438..816
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(472..491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
       61 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
      121 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      301 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      361 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      421 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      481 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      541 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      601 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      661 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      721 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      781 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      901 accacttctg cgctcggccc ttccgg
//
