LOCUS       sequence-83425-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as 41025, with additional linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  TITLE     various
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(35..222)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     143..167
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(304..324)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatctatatt accctgttat ccctagcgga tctgccggta gaggtgtggt caataagagc
       61 gacctcatac tatacctgag aaagcaacct gacctacagg aaagagttac tcaagaataa
      121 gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta ttttttttat
      181 aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag aagtggcgcg
      241 ccaccagcac cgtaatcagc aagtttgtat agttcatcca tgccatgtgt aatcccagca
      301 gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg
      361 gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      421 gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt
      481 ttgaagttaa ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag
      541 ttatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct
      601 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
      661 tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca
      721 ctcttgaaaa agtcatgccg tttcatatga tctgggtatc ttgaaaagca ttgaacacca
      781 taagtgaaag tagt
//
