LOCUS       sequence-83426-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength red-shifted genetically encoded calcium 
            indicator constructed from calmodulin and cp-mRuby.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Akerboom J, Carreras Calderon N, Tian L, Wabnig S, Prigge M, Tolo J,
            Gordus A, Orger MB, Severi KE, Macklin JJ, Patel R, Pulver SR, 
            Wardill TJ, Fischer E, Schuler C, Chen TW, Sarkisyan KS, Marvin JS, 
            Bargmann CI, Kim DS, Kugler S, Lagnado L, Hegemann P, Gottschalk A, 
            Schreiter ER, Looger LL
  TITLE     Genetically encoded calcium indicators for multi-color neural 
            activity imaging and combination with optogenetics.
  JOURNAL   Front Mol Neurosci. 2013;6:2. doi: 10.3389/fnmol.2013.00002. Epub 
            2013 Mar 4.
  PUBMED    23459413
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013"; volume: "6:2. doi"; pages: " 
            10.3389/fnmol.2013.00002. Epub 2013 Mar 4"
COMMENT     Sequence Label: pRSET-RCaMP1h
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..107
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(111..119,120..134)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..218
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggt tctcatcatc
       61 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
      121 acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag tggaataaga
      181 caggtcacgc agtcagagct ataggtcggc tgagctcagc gatcaacacc gaaatgatgt
      241 acccagcgga tggtggtctg cgtggttaca ctcacatggc gctgaaagtt gatggcggcg
      301 gtcacctgtc ctgttctttc gtgaccacct accgctccaa aaagactgtc ggcaacatta
      361 agatgcctgc cattcattac gtcagccacc gtctggagcg cctggaggag agcgataacg
      421 aaatgtttgt cgtacagcgt gaacacgcag ttgccaagtt tgtgggcctg ggtggtggcg
      481 gcggtaccgg agggagcatg aactccctga tcaaggagaa catgcgtatg aaagtggttc
      541 tggaaggctc cgtaaacggc caccagttca aatgcactgg tgaaggcgaa ggcaacccgt
      601 atatgggcac ccagactatg cgtatcaaag tgatcgaggg tggtccgctg ccgtttgcgt
      661 tcgacatcct ggcgacgtcc tttatgtatg gctcccgtac cttcatcaaa tatccgaaag
      721 gcatcccgga tttctttaag cagtccttcc cggaaggttt tacctgggaa cgtgtgaccc
      781 gttacgaaga cggcggcgta attaccgtta tgcaagacac gtctctggag gatggctgcc
      841 tggtgtatca cgtgcaggtt cgcggtgtta acttcccgag caatggtgct gtaatgcaaa
      901 agaaaaccaa aggttgggag cctacggact cccaactgac tgaagagcag atcgcagaat
      961 ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc
     1021 tggggacggt gatgcggtct ctggggcaga
//
