LOCUS       sequence-83427-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as 41026, with additional linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     various
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-GST-LINKER
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..692)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(59..81)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tctggcgcgc caccagcacc gtaatcagca agatccgatt ttggaggatg gtcgccacca
       61 ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac
      121 ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca
      181 tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca
      241 ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt
      301 ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta
      361 tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc
      421 tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat ataacgtatg
      481 atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag attgggaaac
      541 tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg ctcatacaaa
      601 tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg cacaaggccc
      661 ttaattttcc aataacctag tataggggac atattaatta acctccagga tgataaacgg
      721 atcccccgaa ttgatccggt aatttagtgt gtgtatttgt gtttgcgtgt ctatagaagt
      781 atagtaattt atgctacaaa ggacctaatg tataagg
//
