LOCUS       sequence-83429-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     various
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-hphMX-(3×FLAG)-TEV-ProtA
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..116
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             204..377
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             378..551
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      605..792
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(660..684)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcgacggat ccccgggtta attaatatgt ctttaatcaa tatggactac aaggacgacg
       61 atgacaagga ttacaaagat gacgacgata aggactataa ggacgatgat gataaagatt
      121 atgatattcc aactactgct agcgagaatt tgtattttca gggtgagctc aaaaccgcgg
      181 ctcttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa caaaacgcgt
      241 tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa
      301 gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa aagctaaatg
      361 atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac gcgttctatg
      421 agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa
      481 aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatggtgctc
      541 aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac ctgaggcgcg ccacttctaa
      601 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
      661 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
      721 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
      781 cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt tagcttgcct
      841 cg
//
