LOCUS       sequence-83454-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector for enhanced eGFP 
            expression/marking in primary B cells and B-cell lines .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Mock U, Thiele R, Uhde A, Fehse B, Horn S
  TITLE     Efficient lentiviral transduction and transgene expression in 
            primary human B cells.
  JOURNAL   Hum Gene Ther Methods. 2012 Dec;23(6):408-15. doi: 
            10.1089/hgtb.2012.160.
  PUBMED    23240650
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2012";
            journalName: "Hum Gene Ther Methods"; date: "2012-12"; volume: "23";
            issue: "6"; pages: "408-15"
COMMENT     Sequence Label: LeGO-hEµMAR-G2
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(42..448)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ggtggcggga tccgggcgac tcagtcaatc
       61 ggaggactgg cgcgccgagt gaggggttgt gagctctttt atagagctcg ggaagcagaa
      121 gcgcgcgaac agaagcgaga agcaggctga ttggttaatt caaataaggc gcagggtcat
      181 ttcaggtcct tgggggagcc tggaaacatc tgatgggtct taagaaactg ctgagggttg
      241 ggccatatct ggggaccatc tgttcttggc ctcgggccgg ggccgaaact gcggtgacca
      301 tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg cagatatcct gtttggccca
      361 acgttagcta ttttcatgta cccgcccttg atctgaactt ctctattctt ggtttggtat
      421 ttttccatgc cttgcaaaat ggcgttactg cagctagcta tctgattgag ttttctctta
      481 tgccaccaac taacatgctt aactgaaata attcaggata atgatgcaca ttttacctaa
      541 aacttatcct aaagtgagta gttgaaaagt ggtcttgaaa aatactaaaa tgaaggccac
      601 tctatcagaa tatcaaagtg tttctcctta atcacaaaga gagaacgagt taacctaaaa
      661 agattgtgaa cacagtcatt atgaaaataa tgctctgagg tatcgaaaaa gtatttgaga
      721 ttagttatca catgaaggga taacaagcta atttaaaaaa ctttttgaat acagtcataa
      781 actctcccta agactgttta atttcttaaa catcttactt taaaaatgaa tgcagtttag
      841 aagttgatat gctgtttgca caaactagca gttgataagc taagattgga aatgaaattc
      901 agatagttaa aaaaagcctt ttcagtttcg gtcagcctcg ccttatttta ga
//
