LOCUS       sequence-83456-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector for enhanced transgene and 
            eGFP-coexpression in primary B cells and B-cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Mock U, Thiele R, Uhde A, Fehse B, Horn S
  TITLE     Efficient lentiviral transduction and transgene expression in 
            primary human B cells.
  JOURNAL   Hum Gene Ther Methods. 2012 Dec;23(6):408-15. doi: 
            10.1089/hgtb.2012.160.
  PUBMED    23240650
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2012";
            journalName: "Hum Gene Ther Methods"; date: "2012-12"; volume: "23";
            issue: "6"; pages: "408-15"
COMMENT     Sequence Label: LeGO-hEµMAR-iG2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(160..162,163..165,166..879)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(160..162,163..165,166..876)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(205..226)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(466..485)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     813..834
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
       61 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
      121 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag
      181 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      241 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      301 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      361 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      421 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      481 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      541 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      601 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      661 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      721 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      781 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      841 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccggcc gccagcacag
//
