LOCUS       sequence-83457-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector for enhanced transgene and 
            eGFP-coexpression in primary B cells and B-cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Mock U, Thiele R, Uhde A, Fehse B, Horn S
  TITLE     Efficient lentiviral transduction and transgene expression in 
            primary human B cells.
  JOURNAL   Hum Gene Ther Methods. 2012 Dec;23(6):408-15. doi: 
            10.1089/hgtb.2012.160.
  PUBMED    23240650
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2012";
            journalName: "Hum Gene Ther Methods"; date: "2012-12"; volume: "23";
            issue: "6"; pages: "408-15"
COMMENT     Sequence Label: LeGO-hEµMAR-iG2
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(59..92)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
       61 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgg
      121 ccgctgaaag accccacctg taggtttggc aagctagcag gattgtttat cttaggaggc
      181 atgcttactg ttaaaagaca ggatatgttt gaagtggctt ctgagaaaaa tggttaagaa
      241 aattatgact taaaaatgtg agagattttc aagtatatta atttttttaa ctgtccaagt
      301 atttgaaatt cttatcattt gattaacacc catgagtgat atgtgtctgg aattgaggcc
      361 aaagcaagct cagctaagaa atactagcac agtgctgtcg gccccgatgc gggactgcgt
      421 tttgaccatc ataaatcaag tttatttttt taattaattg agcgaagctg gaagcagatg
      481 atgaattaga gtcaagatgg ctgcatgggg gtctccggca cccacagcag gtggcaggaa
      541 gcaggtcacc gcgagagtct attttaggaa gcaaaaaaac acaattggta aatttatcac
      601 ttctggttgt gaagaggtgg ttttgcccag gcccagatct gaaagtgctc tactgagcaa
      661 aacaacacct ggacaatttg cgtttctaaa ataaggcgag gctgaccgaa actgaaaagg
      721 ctttttttaa ctatctgaat ttcatttcca atcttagctt atcaactgct agtttgtgca
      781 aacagcatat caacttctaa actgcattca tttttaaagt aagatgttta agaaattaaa
      841 cagtcttagg gagagtttat gactgtattc aaaaagtttt ttaaattagc ttgttatccc
      901 ttcatgtgat aactaatctc aaatactttt t
//
