LOCUS       sequence-83460-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the FlpO 
            recombinase gene in neurons from the synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1(S)-FlpO-bGHpA
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..83
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgtcgtgcc tgagagcgca gacgcgtgga cggtaccaag cttacgcgta gccaccatgg
       61 ctcctaagaa gaagaggaag gtgatgagcc agttcgacat cctgtgcaag acccccccca
      121 aggtgctggt gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca
      181 gctgtgccgc cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca
      241 agagggccac cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg
      301 tgaacaagag cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc
      361 tgaagaagct gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc
      421 agagcgacat caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg
      481 ccgacaaggg caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga
      541 gcatctggga gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca
      601 agaccaagac cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca
      661 gcgacatcaa gaacgtggac ccc
//
