LOCUS       sequence-83474-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dominant-negatively inhibits RFX (regulatory factor X) transcription
            factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and 
            C-terminal myc tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang 
            PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, 
            Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh 
            CA
  TITLE     Evolutionarily dynamic alternative splicing of GPR56 regulates 
            regional cerebral cortical patterning.
  JOURNAL   Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
  PUBMED    24531968
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-14- 14"; volume: "343"; 
            issue: "6172"; pages: "764-8"
COMMENT     Sequence Label: pCAG-DN-RFX
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             413..442
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(535..554)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    600..655
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(654..673)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ccgatatcgc ggccgcgcca ccatggaaat ccaaggcggc tacatgctag gcaatgccag
       61 ccagtcttac tcccacacca cccgtgcctc accagccaca gtccagtggc tcctggataa
      121 ttacgagacc gctgagggcg tgagcctgcc acggagcacc ctctactgcc actacctgct
      181 gcactgccaa gagcagaagc tggagccagt taatgccgcc tctttcggca agctcatccg
      241 ctcggtattc atgggtctgc gcacgcgccg tctgggcacc aggggcaact ctaagtacca
      301 ctattacggc ctgcggatca aagccagctc acccctgctg cggctgatgg aggaccagca
      361 gcacatggcc atgcggggcc agcccttctc ccagaaacag aggctgaagc ccgaacaaaa
      421 actcatctca gaagaggatc tgtaactcga gggccgcact cctcaggtgc aggctgccta
      481 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
      541 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
      601 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
      661 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
      721 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
      781 gtatatgaaa cagccccc
//
