LOCUS       sequence-83477-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express Cre in neurons 
            from the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pmSyn1-EBFP-Cre
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(116..826,827..829,830..832))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     766..787
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      829..838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt gcatcgaccg
       61 gtaatgcagg caaattttgg tgtacggtca gtaaattggc catggaaccg gaacccttgt
      121 acagctcgtc catgccgaga gtgatcccgg cggcggtgcg gaactccagc aggaccatgt
      181 gatcgcgctt ctcgttgggg tctttgctca gcacggactg ggtgctcagg tagtggctgt
      241 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      301 gcacgctgcc gtcctccacg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
      361 gcttgacggc catgatatag atgttgtggc tgttgaagtt gtactccagc ttgtgcccca
      421 ggatgttgcc gtcctccttg aagtcgacgc ccttcagctc gatgcggttc actagggtgt
      481 cgccctcgaa cttcacctcg gcgcgggtct tgtaggtgcc gtcgtccttg aagaagatgg
      541 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      601 ggtcggggta gcgggcgaag cactgcacgc cgtggctcag ggtggtcacg agggtgggcc
      661 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgttggtgg
      721 catcgccctc gccctcgccc ctcacgctga acttgtggcc gtttacgtcg ccgtccagct
      781 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga
      841 ccggtgcaga tcggggaatt ccaaggggca gtgggtctgt gggccgcggg ggcgga
//
