LOCUS       sequence-83478-e        5271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an active HBFD (carotenoid 4-hydroxy-beta-ring 
            4-dehydrogenase) enzyme in E. coli when expression is induced with 
            arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     Elucidation of the pathway to astaxanthin in the flowers of Adonis 
            aestivalis.
  JOURNAL   Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. 
            Epub 2011 Aug 23.
  PUBMED    21862704
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.086827"; journalName: "Plant Cell"; date: 
            "2011-08"; volume: "23"; issue: "8"; pages: "3055-69"
COMMENT     Sequence Label: pHBFD1Bad
FEATURES             Location/Qualifiers
     source          1..5271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1382..1393
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(1672..1689)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1842..1928
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2020..2047
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2066..2157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2158..2226,2227..3018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2376..2395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3189..3777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3678..3697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3931..3948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3963..4103
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4029..4048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4189..4211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4367..5245)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggctcctgtt ctccttggat tgaaaccaac tctctccact
      361 ggaagcgtcg tcaaagagac taatgtagga agcacacttg ctagtcccct taacaaaacc
      421 cagaattcaa gggttttggt tttgggcgga acagggaagg tcggtggttc cacagctttg
      481 gctctctcca agttctcacc tgacctcagg cttgtgattg gaggtcgaaa cagggagaaa
      541 ggtgatgctg tagtgtctaa actaggagaa aactccgagt ttgttgaagt caacgttgac
      601 agtgtgagat ctttagaatc tgctctcgaa gatgtggacc ttgtagttca tgcagctgga
      661 ccttttcaac aagcggagaa gtgcactgtt ctagaagctg caatatctac caggacggcc
      721 tatgtggatg tatgtgataa tacaagttat tccatgcaag caaagtcttt tcatgataaa
      781 gcagtggctg ccaacgttcc tgccataaca actgctggaa ttttccctgg agtgagcaat
      841 gtgatagcag ctgagctagt gcgatcagca agagatgaaa acactgaacc tcaaagacta
      901 agattctcct attttaccgc gggttctggt ggtgctggtc caacgtcgtt agttactagc
      961 ttcttgcttc ttggtgaaga ggttgttgct tacagtgaag gcgaaaaagt cgaattaaag
     1021 ccttatacag ggaagcttaa cattgacttc gggaagggag ttgggaaaag agacgtttat
     1081 ttgtggaact tgccggaagt aagaagtggt catgagatct taggagtacc aactgtgagt
     1141 gctcgattcg gtactgcacc tttcttctgg aattgggcga tggtagctat gacaactctc
     1201 cttcctcctg gtattctgag agacagaaat aaaatcggaa tgttggcaaa ttttgtgtac
     1261 ccttctgtac aaatttttga tgggattgca ggagaatgtc ttgcaatgcg ggttgattta
     1321 gagtgcgcaa atgggcgcaa tacttttggt atactcagtc atgaacgtct ctctgtatta
     1381 gtgggaactt caactgcggt gtttgctatg gcaattcttg aaggaagtac gcagcctgga
     1441 gtttggtttc cagaagagcc tggagggatt gcaataagtg acagagagtt acttctacaa
     1501 cgagcatcac aaggagcgat taacttcatt atgaagcagt agagtaatag attggattat
     1561 tccttatgta gcccagatct gaattcgtcg acaagcttct cgagatctgc agctggtacc
     1621 atatgggaat tcgaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     1681 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1741 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     1801 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     1861 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1921 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     1981 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     2041 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2401 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3301 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3961 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     4021 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     4081 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     4141 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4201 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
     4261 cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
     4321 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
     4381 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     4441 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     4501 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     4561 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     4621 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     4681 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     4741 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     4801 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     4861 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     4921 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     4981 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     5041 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     5101 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     5161 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     5221 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga g
//
