LOCUS       sequence-83489-e        9948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral destination vector for C-Bio-1xproteinA constructs using 
            Gateway recombination System. Design for cDNA clones from the 
            CCSBHuman Orfeome Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pHAGE- TRE-DEST-CBioTAP
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2740..2758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2761..2779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2789..2813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2921..3045
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3070..3075,3076..3093,3094..3100)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3154..3813
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3220..3239)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4155..4460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4339..4358
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4501..4625)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4900..5073
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             5093..5119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5152..5651
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5187..5210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5584..5603
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5672..6271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5672..5691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6168..6188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6338..6926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6391..6411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6809..6820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7001..7234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7353..7371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7439..7543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7544..7612,7613..8404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7762..7781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8575..9163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9064..9083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9317..9334
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9574..9595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9610..9615,9616..9633,9634..9640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9648..9664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9653..9675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9672..9688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9672..9688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9738..9757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggcta
     2221 gcatgcatgt cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     2761 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
     2821 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     2881 ctctagagga tccctaccgg tgatatcctc gagcccatca acaagtttgt acaaaaaagc
     2941 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3001 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3061 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3121 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     3301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     3361 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     3421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     3481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     3541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     3661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     3721 caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     3781 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     3841 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3901 tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg
     3961 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4021 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4081 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4141 cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4201 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     4261 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     4321 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     4381 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4441 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     4501 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4561 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4621 gtggtttgcg caaagctagg aagccaagag ggcccagctg gaaaggccgg tgaaggtgaa
     4681 atccctgccc ctcttgctgg taccgtttct aagatactgg taaaagaagg tgacactgtt
     4741 aaagctggtc aaacagttct ggtgctggag gctatgaaaa tggagacaga aattaacgct
     4801 cctactgacg gaaaagttga aaaggtgtta gttaaggaaa gagatgctgt tcaaggtggt
     4861 caaggtctaa tcaagatcgg cgttggatcg tcactcgagg tggacaacaa attcaacaaa
     4921 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
     4981 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
     5041 gctaaaaagc taaatggtgc tcaggcgccg aaagtataat aggcggttcg aatacccata
     5101 cgacgtccca gactacgctt agtaatgatt aattaaacta gaaattctac cgggtagggg
     5161 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     5221 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     5281 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     5341 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     5401 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     5461 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     5521 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     5581 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     5641 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     5701 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5761 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5821 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5881 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5941 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6001 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6061 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6121 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6181 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6241 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     6301 cgaaaggagc gcacgacccc atgcatcgat agatcctaat caacctctgg attacaaaat
     6361 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6421 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6481 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6541 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6601 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6661 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6721 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6781 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6841 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6901 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     6961 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7021 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7081 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7141 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7201 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7261 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     7321 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     7381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     7801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9361 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt
     9421 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9481 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc
     9541 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9601 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9661 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt
     9721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9781 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9841 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9901 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
