LOCUS       sequence-83493-e        3883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector carrying N-Bio-1xproteinA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pCAndy-NBioTAP
FEATURES             Location/Qualifiers
     source          1..3883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(617..1276)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1191..1210
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1277..1379)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1599..2187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2088..2107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2341..2358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2475..2496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2511..2516,2517..2534,2535..2541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2549..2565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2554..2576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2573..2589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2573..2589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2608..2628
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2610..2628
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2697..2870
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     primer_bind     complement(3198..3214)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3199..3215)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3239..3258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3240..3258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3268..3285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3268..3284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3277..3299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3426..3881
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3513..3532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3723..3744
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac ccttgataaa tgcttcaata atattgaaaa
      121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      301 tttcgccccg aagaacgttt tccaatgatg agcacttttg atcagaaaaa aaggatcata
      361 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
      421 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      481 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
      541 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
      601 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      661 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      721 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      781 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      841 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      901 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      961 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1021 cccatatcac cagctcaccg tctttcattg ccatacgtaa ttccggatga gcattcatca
     1081 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1141 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1201 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1261 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacaac tcaaaaaata
     1321 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1381 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1441 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggtcgaa aaggatctag
     1501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1741 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2221 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2281 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2341 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2401 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2461 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2521 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2581 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     2641 gagctccacc gcggtggcgg ccgcccatgg caggccttgc gcaacacgat gaagccgtgg
     2701 acaacaaatt caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact
     2761 taaacgaaga acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg
     2821 ctaacctttt agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacgcga
     2881 attgtgatat acctacaact gcttctctcg agagcggctc ctcggctgga aaggccggtg
     2941 aaggtgaaat ccctgcccct cttgctggta ccgtttctaa gatactggta aaagaaggtg
     3001 acactgttaa agctggtcaa acagttctgg tgctggaggc tatgaaaatg gagacagaaa
     3061 ttaacgctcc tactgacgga aaagttgaaa aggtgttagt taaggaaaga gatgctgttc
     3121 aaggtggtca aggtctaatc aagatcggcg ttcgaattcg gcgcgccgat atccagatct
     3181 tttaattaaa agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     3241 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3301 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3421 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3481 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3661 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3721 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3781 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3841 atttaacgcg aattttaaca aaatattaac gcttacaatt tag
//
