LOCUS       sequence-83525-e        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: VSV.G
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..170
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     polyA_signal    227..348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(238..257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     292..311
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      529..984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(616..635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     826..847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1051..1070)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc atagggttaa
       61 tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa ttaaagcaca
      121 ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag taactcaaat
      181 cctgcactct agagtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
      241 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      301 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      361 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
      421 aacctctaca aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc
      481 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
      541 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      601 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      661 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      721 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      781 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      841 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      901 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      961 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     1021 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     1081 ccagccccga cacccgccaa cacccgctga cg
//
