LOCUS       sequence-83528-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wt Ulk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pLJM60 mUlk1 (wt) barcoded
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        248..266
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     248..265
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     279..298
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             357..380
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggtacc gatccagcct ccgcggccgg gaacggtgca
      121 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      181 ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac
      241 acatacgatt taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt
      301 ccactcccag gtccaactgc acctcggttc tatcgattga attcaccatg gaaatggact
      361 acaaggatga cgatgacaag ggtggtggtg gtggtgcgtc gaggggatcc atggagccgg
      421 gccgcggcgg cgtcgagacc gtgggcaagt tcgagttctc tcgcaaggac ctgattggac
      481 acggcgcctt cgcggtggtc ttcaagggtc gacaccgcga gaagcacgac ctggaggtgg
      541 ccgtcaaatg cattaacaag aagaaccttg ccaagtccca aacactgctg ggaaaggaaa
      601 tcaaaatcct gaaggaacta aagcacgaaa acatcgtggc gctgtatgac ttccaggaaa
      661 tggctaattc tgtctacctg gtcatggagt attgtaatgg tggagacctg gctgactacc
      721 tgcacactat gcgcacactg agtgaagaca ctgtcaggct tttcctacag cagatcgctg
      781 gcgccatgcg gctgctgcac agcaagggca tcatccaccg ggacctgaag ccccaaaaca
      841 tcctgctgtc caaccctggg ggccgccggg ccaaccccag caacatccga gtcaagattg
      901 ctgactttgg attcgctcgg tacctccaga gcaacatgat ggcggccaca ctctgtggtt
      961 ctcctatgta catggctcct gaggtcatta tgtcccagca ctacgatgga aaggctgacc
     1021 tgtggagcat tggcaccatt gtctaccagt gtctgacagg gaaggcccct tttcaggcca
     1081 gcagccctc
//
