LOCUS       sequence-83529-e        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mutant Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pRK5 HA-GST-Presc-mAkt1 (S473T)
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        198..216
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     198..215
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     229..248
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      333..342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             345..371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             402..1055
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1013..1035
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1077..1100
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
       61 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
      121 gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta
      181 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
      241 ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga attcccgaac
      301 cgacagtcgg tctcttcacc aaggccattc gcgccaccat gggctacccc tatgatgtgc
      361 ctgactacgc agatctcaat ggtggtggtg gtgggtcgag aatgtcccct atactaggtt
      421 attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa
      481 aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg
      541 aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac
      601 agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa
      661 aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt
      721 cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac
      781 ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc
      841 atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc
      901 caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc
      961 cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc
     1021 aagccacgtt tggtggtggc gaccatcctc caaaaggtgg aggtggtggg tcgagtctgg
     1081 aagttctgtt ccaggggccc tggtcgacaa tgaacgacgt agccattgtg aaggagggct
     1141 ggc
//
