LOCUS       sequence-83530-e        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses mutant Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pRK5 HA-GST-Presc-mAkt1 (S473T)
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..90)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(187..206)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(219..237)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(220..237)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(447..471)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(468..671)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(497..517)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        672..1051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1080..1096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1080..1096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1093..1115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1104..1120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cttaattttc caataaccta gtatagggga cattctcgac ccaccaccac caccattgag
       61 atctgcgtag tcaggcacat cataggggta gcccatggtg gcgcgaatgg ccttggtgaa
      121 gagaccgact gtcggttcgg gaattcaatc gatagaaccg aggtgcagtt ggacctggga
      181 gtggacacct gtggagagaa aggcaaagtg gatgttattc tatagtgtca cctaaatcgt
      241 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga agccaagggg
      301 gtgggcctat agactctata ggcggtactt acgtcactct tggcacgggg aatccgcgtt
      361 ccaatgcacc gttcccggcc gcggaggctg gatcggtccc ggtgtcttct atggaggtca
      421 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata
      481 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      541 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      601 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      661 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      721 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      781 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      841 aaatactcca cacattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      901 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      961 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     1021 tactattaat aactagtcaa taatcaatgt cgggcgagct cgaattaatt cgtaatcatg
     1081 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1141 ggaagcataa ag
//
