LOCUS       sequence-83551-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Receiving vector for PUM-HD assembly and expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pCMV-TTP(C147R)-GG-PUF
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    187..208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(223..228,229..246,247..253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    261..277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     266..288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     285..301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     285..301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             297..623
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRFKQSTLDLIKDPARPRVPSSNSLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGVSSGN
                     LPQL"
     primer_bind     complement(398..415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(398..414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(407..429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcactgggga ggggtcacag ggatgccacc cgggatcctc tagagtcgac ttaccctaag
       61 tcaacaccgt tcttcatgta gtacttctcc agcttggcca gaatgtgctt gccataggtg
      121 tacttacgaa gagttgcgat gtggggttga gaccgactgg aaagcgggca gtgagcgcaa
      181 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      241 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      301 ccatgattac gccaagcttg catgcctgca ggtttaaaca gtcgactcta gacttaatta
      361 aggatccggc gcgcccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc
      421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggggtctctt
      601 ccggcaatct cccgcagttg taaattgggg taccggttgt ttcgaaaatc ttccaaaagg
      661 cggctgcggc ccccaccgcc tccagatcca ccgccacctg aaccgccacc tccgcttcca
      721 ccacctccct cagaaacaga gatgcgattg aagatgggga gtcgccgggg ggctgccacg
      781 ggctggggtg gtgcaaaaac tcccgcctcg aagacgggag agtcagagcc ccccaggctg
      841 ctgccgctgc tggcatattc atcagggtcg gatcccaggg actgtacaga gggggtccga
      901 accaggccac ccaagggccc
//
