LOCUS       sequence-83559-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 
            2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-7SYR
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(232..248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(232..248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(245..267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    256..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(280..286,287..304,305..310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    325..346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(463..480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(714..733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
       61 gagctcggtc tcaaatgtac ttgtattgag tcagcacaaa tttgcaagct atgttgtgcg
      121 caagtgtgtt actcacgcct cacgtacgga gcgcgctgtg ctcatcgatg aggtgtgcac
      181 catgaacgac ggtccccaca gtgcctagag accaagcttg gcgtaatcat ggtcatagct
      241 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      421 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      661 gcatcacaaa aatcgacgct caagtcagag gtggtgaaac ccgacaggac tataaagata
      721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt c
//
