LOCUS       sequence-83566-e        1141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hTTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1141)
  TITLE     pFRT-TODestFLAGHAhTTP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhTTP
FEATURES             Location/Qualifiers
     source          1..1141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(163..231,232..1023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(381..400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc
       61 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1141 g
//
