LOCUS       sequence-83586-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expresses WT hTIRAPfused to GFP. Also contains 
            IRES-hCD2 as selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, 
            Iwasaki A, Knipe DM, Kagan JC
  TITLE     A promiscuous lipid-binding protein diversifies the subcellular 
            sites of toll-like receptor signal transduction.
  JOURNAL   Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019.
  PUBMED    24529375
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "705-16"
COMMENT     Sequence Label: TIRAP-GFP
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(835..856)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctctcg agctcaagct tatggcatca
       61 tcgacctccc tcccagctcc tggctctcgg cctaagaagc ctctaggcaa gatggctgac
      121 tggttcaggc agaccctgct gaagaagccc aagaagaggc ccaactcccc agaaagcacc
      181 tccagcgatg cttcacagcc tacctcacag gacagcccac tacccccaag cctcagctca
      241 gtcacgtctc ccagcctgcc acccacacat gcgagtgaca gtggcagtag tcgctggagc
      301 aaagactatg acgtctgcgt gtgccacagt gaggaagacc tggtggccgc ccaggacctg
      361 gtctcctact tggaaggcag cactgccagc ctgcgctgct tcctgcaact ccgggatgca
      421 accccaggcg gcgctatagt gtccgagctg tgccaggcac tgagcagtag tcactgccgg
      481 gtgctgctca tcacgccggg cttccttcag gacccctggt gcaagtacca gatgctgcag
      541 gccctgaccg aggctccagg ggccgagggc tgcaccatcc ccctgctgtc gggcctcagc
      601 agagctgcct acccacctga gctccgattc atgtactacg tcgatggcag gggccctgat
      661 ggtggctttc gtcaagtcaa agaagctgtc atgcgttgta agctactaca ggagggagaa
      721 ggggaacggg attcagctac agtatctgat ctactggtac cgcgggcccg ggatccaccg
      781 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tgg
//
