LOCUS       sequence-83588-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expresses hTIRAP-3xHA and a target site for the 
            biotin ligase BirA upstream of IRES and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, 
            Iwasaki A, Knipe DM, Kagan JC
  TITLE     A promiscuous lipid-binding protein diversifies the subcellular 
            sites of toll-like receptor signal transduction.
  JOURNAL   Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019.
  PUBMED    24529375
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "705-16"
COMMENT     Sequence Label: TIRAP-TAP_IRES_GFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             761..841
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             842..862
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             878..898
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             914..934
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atctgccgcc accatggcat catcgacctc
       61 cctcccagct cctggctctc ggcctaagaa gcctctaggc aagatggctg actggttcag
      121 gcagaccctg ctgaagaagc ccaagaagag gcccaactcc ccagaaagca cctccagcga
      181 tgcttcacag cctacctcac aggacagccc actaccccca agcctcagct cagtcacgtc
      241 tcccagcctg ccacccacac atgcgagtga cagtggcagt agtcgctgga gcaaagacta
      301 tgacgtctgc gtgtgccaca gtgaggaaga cctggtggcc gcccaggacc tggtctccta
      361 cttggaaggc agcactgcca gcctgcgctg cttcctgcaa ctccgggatg caaccccagg
      421 cggcgctata gtgtccgagc tgtgccaggc actgagcagt agtcactgcc gggtgctgct
      481 catcacgccg ggcttccttc aggacccctg gtgcaagtac cagatgctgc aggccctgac
      541 cgaggctcca ggggccgagg gctgcaccat ccccctgctg tcgggcctca gcagagctgc
      601 ctacccacct gagctccgat tcatgtacta cgtcgatggc aggggccctg atggtggctt
      661 tcgtcaagtc aaagaagctg tcatgcgttg taagctacta caggagggag aaggggaacg
      721 ggattcagct acagtatctg atctacttag atctctcgag tatccttacg atgttcctga
      781 ttatgcttac ccctacgacg tccccgacta cgcctatcca tacgatgtac cagactatgc
      841 ggaaaatctt tattttcaag gtgctattcc tactactgag aacctctact tccagggcgc
      901 catccccaca acggaaaacc tatatttcca aggaggg
//
