LOCUS       sequence-83589-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      46..181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    221..342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(232..251)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     286..305
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             420..653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             831..842
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc
       61 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
      121 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
      181 tgggcggagt taggggcggg atagctagag ccagacatga taagatacat tgatgagttt
      241 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      301 attgctttat ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat
      361 tcatttcttt gcaagttata aatactgaat aataagatga catgaactac tatacgtact
      421 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
      481 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      541 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      601 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
      661 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagt
      721 cgacgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
      781 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
      841 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
      901 c
//
