LOCUS       sequence-8359-ex        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2T
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(685..709)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      776..823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgtttaact ttaagaagga ganatacata tgtcgtacta ccatcaccat caccatcacc
       61 tcgaatcaac aagtttgtac aaaaaagttg ggatgcagag agcttcacgt ctgaagagag
      121 agctgcacat gttagccaca gagccacccc caggcatcac atgttggcaa gataaagacc
      181 aaatggatga cctgcgagct caaatattag gtggagccaa cacaccttat gagaaaggtg
      241 tttttaagct agaagttatc attcctgaga ggtacccatt tgaacctcct cagatccgat
      301 ttctcactcc aatttatcat ccaaacattg attctgctgg aaggatttgt ctggatgttc
      361 tcaaattgcc accaaaaggt gcttggagac catccctcaa catcgcaact gtgttgacct
      421 ctattcagct gctcatgtca gaacccaacc ctgatgaccc gctcatggct gacatatcct
      481 cagaatttaa atataataag ccagccttcc tcaagaatgc cagacagtgg acagagaagc
      541 atgcaagaca gaaacaaaag gctgatgagg aagagatgct tgataatcta ccagaggctg
      601 gtgactccag agtacacaac tcaacacaga aaaggaaggc cagtcagcta gtaggcatag
      661 aaaagaaatt tcatcctgat gtttacccaa ctttcttgta caaagtggtt gattcgaggc
      721 tgctaacaaa gcccgaaagg aagctgantt ggctgctgcc accgctganc aataactagc
      781 ataaccnctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      841 atccgganat ccacaggacg ggtgtggtcg ccatgatcgc gtantcgata gtggctccaa
      901 gtancgaanc gagcagnant gggcggcggc caaagcggtc gganagtgct ccnanaacgg
      961 nngcncatan aaattgcatc aacgcatata gcgctagcag cacgccnnan tgactggnga
     1021 tgcngnngga anggacna
//
