LOCUS       sequence-83609-e        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt1
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..72
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1135..1161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1141..1167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 atcgataagc ttgatatcga attcgccgcc atggggagta gcaagagcaa gcctaaggac
       61 cccagccagc gcagcgaggc tgaagagatg gaggtgtccc tggccaagcc caagcaccgc
      121 gtgaccatga acgagtttga gtacctgaag ctgctgggca agggcacttt cggcaaggtg
      181 atcctggtga aggagaaggc cacaggccgc tactacgcca tgaagatcct caagaaggaa
      241 gtcatcgtgg ccaaggacga ggtggcccac acactcaccg agaaccgcgt cctgcagaac
      301 tccaggcacc ccttcctcac agccctgaag tactctttcc agacccacga ccgcctctgc
      361 tttgtcatgg agtacgccaa cgggggcgag ctgttcttcc acctgtcccg ggaacgtgtg
      421 ttctccgagg accgggcccg cttctatggc gctgagattg tgtcagccct ggactacctg
      481 cactcggaga agaacgtggt gtaccgggac ctcaagctgg agaacctcat gctggacaag
      541 gacgggcaca ttaagatcac agacttcggg ctgtgcaagg aggggatcaa ggacggtgcc
      601 accatgaaga ccttttgcgg cacacctgag tacctggccc ccgaggtgct ggaggacaat
      661 gactacggcc gtgcagtgga ctggtggggg ctgggcgtgg tcatgtacga gatgatgtgc
      721 ggtcgcctgc ccttctacaa ccaggaccat gagaagcttt ttgagctcat cctcatggag
      781 gagatccgct tcccgcgcac gcttggtccc gaggccaagt ccttgctttc agggctgctc
      841 aagaaggacc ccaagcagag gcttggcggg ggctccgagg acgccaagga gatcatgcag
      901 catcgcttct ttgccggtat cgtgtggcag cacgtgtacg agaagaagct cagcccaccc
      961 ttcaagcccc aggtcacgtc ggagactgac accaggtatt ttgatgagga gttcacggcc
     1021 cagatgatca ccatcacacc acctgaccaa gatgacagca tggagtgtgt ggacagcgag
     1081 cgcaggcccc acttccccca gttctcctac tcggccagca gcacggccat ggcttatcct
     1141 tacgacgtcc ccgactacgc cagtctggga cctggactgt ga
//
