LOCUS       sequence-83624-e        4765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase
            in E. coli. Use with pAC-LYC or pAC-LYCipi to produce 
            epsilon-carotene in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     One ring or two? Determination of ring number in carotenoids by 
            lycopene epsilon-cyclases.
  JOURNAL   Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2905-10. Epub 2001 Feb 
            20.
  PUBMED    11226339
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2001-02-27"; volume: "98(5)"; pages: 
            "2905-10. Epub 2001 Feb 20"
COMMENT     Sequence Label: pDY4
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2223..2239
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4080..4096)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4081..4097)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4121..4140)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4122..4140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4147..4164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4147..4163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4156..4178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4305..4760
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4392..4411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4602..4623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtggatcc cccgggctgc aggaattcgc ggccgctgaa acaaatgacg
     2281 tgaaagttct tcaaaattga attaattgta atcctgaaaa cttgatttgt gatagaagaa
     2341 tcaatggagt gctttggagc tcgaaacatg acggcaacaa tggcggtttt tacgtgccct
     2401 agattcacgg actgtaatat caggcacaaa ttttcgttac tgaaacaacg aagatttact
     2461 aatttatcag catcgtcttc gttgcgtcaa attaagtgca gcgctaaaag cgaccgttgt
     2521 gtagtggata aacaagggat ttccgtagca gacgaagaag attatgtgaa ggccggtgga
     2581 tcggagctgt tttttgttca aatgcagcgg actaagtcca tggaaagcca gtctaaactt
     2641 tccgaaaagc tagcacagat accaattgga aattgcatac ttgatctggt tgtaatcggt
     2701 tgtggccctg ctggccttgc tcttgctgca gagtcagcca aactagggtt gaacgttgga
     2761 ctcattggcc ctgatcttcc ttttacaaac aattatggtg tttggcagga tgaatttata
     2821 ggtcttggac ttgaaggatg cattgaacat tcttggaaag atactcttgt ataccttgat
     2881 gatgatgctg atcccatccg cataggtcgt gcatatggca gagttcatcg tgatttactt
     2941 catgaagagt tgttaagaag gtgtgtggaa tcaggtgttt catatctaag ctccaaagta
     3001 gaaagaatca ctgaagctcc aaatggctat agtctcattg aatgtgaagg caatatcacc
     3061 attccatgca ggcttgctac tgttgcatca ggggcagctt cagggaaatt tctggagtat
     3121 gaacttgggg gtccccgtgt ttgtgtccaa acagcttatg gtatagaggt tgaggttgaa
     3181 aacaacccct atgatccaga tctaatggtg ttcatggatt atagagactt ctcaaaacat
     3241 aaaccggaat ctttagaagc aaaatatccg actttcctct atgtcatggc catgtctcca
     3301 acaaaaatat tcttcgagga aacttgttta gcttcaagag aagccatgcc tttcaatctt
     3361 ctaaagtcca aactcatgtc acgattaaag gcaatgggta tccgaataac aagaacgtac
     3421 gaagaggaat ggtcgtatat ccccgtaggt ggatcgttac ctaatacaga acaaaagaat
     3481 ctcgcatttg gtgctgcagc tagtatggtg caccctgcca cagggtattc agttgttcga
     3541 tctttgtcag aagctcctaa ttatgcagca gtcattgcta agattttaag acaagatcaa
     3601 tctaaagaga tgatttctct tggaaaatac actaacattt caaaacaagc atgggaaaca
     3661 ttgtggccac ttgaaaggaa aagacagcga gccttctttc tattcggact atcacacatc
     3721 gtgctaatgg atctagaggg aacacgtaca tttttccgta ctttctttcg tttgcccaaa
     3781 tggatgtggt ggggattttt ggggtcttct ttatcttcaa cggatttgat aatatttgcg
     3841 ctttatatgt ttgtgatagc acctcacagc ttgagaatgg aactggttag acatctactt
     3901 tctgatccga caggggcaac tatggtaaaa gcatatctca ctatatagat ttagattata
     3961 taaataatac ccatatcttg catatatata agccttattt atttcttttg tatccttaca
     4021 acaacatact cgttaattat atgtttttta agcggccgcg aattcgatat caagcttatc
     4081 gataccgtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta
     4141 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4201 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4261 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     4321 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4381 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4441 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4501 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4561 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4621 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4681 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4741 aatattaacg cttacaattt aggtg
//
