LOCUS       sequence-83629-e        4751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme
            in E. coli. Also known as pAt9SK..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Cunningham FX Jr, Lee H, Gantt E
  TITLE     Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon 
            merolae.
  JOURNAL   Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.
  PUBMED    17085635
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2007-03"; volume: "6(3)"; pages: "533-45. Epub 2006 
            Nov 3"
COMMENT     Sequence Label: pAtLCYbSK
FEATURES             Location/Qualifiers
     source          1..4751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2226..2242
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2226..2242
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4066..4082)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4067..4083)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4107..4126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4108..4126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4136..4153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4136..4152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4145..4167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4293..4748)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4430..4451)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4642..4661
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatggatac tctgttgaaa
     2281 acacccaaca agctcgattt tttcatccct cagtttcatg ggtttgagag attatgcagt
     2341 aacaatccat accattcaag ggttaggctt ggtgtgaaga aaagggctat caaaattgtc
     2401 tctagtgtag tgagtggtag cgctgctctt ttggatcttg ttcctgaaac taagaaggag
     2461 aatcttgact ttgagcttcc tttgtacgac acttccaaga gtcaagttgt tgatttggct
     2521 attgttggtg gtggtcctgc tggtttagcc gtggctcagc aggtttctga agctggactc
     2581 tctgtttgtt ccattgatcc ttctcctaag ctcatatggc ctaacaatta tggagtttgg
     2641 gttgatgagt ttgaggctat ggatttacta gactgcctgg ataccacatg gtctggtgct
     2701 gttgtctatg tcgatgaagg tgtcaagaag gatttgagcc ggccttatgg gagagttaac
     2761 cggaaacagc tcaaatccaa aatgcttcag aaatgtatta ccaacggtgt taaatttcat
     2821 cagtctaagg tcactaatgt ggttcacgag gaggcaaact ccactgtggt ctgcagtgac
     2881 ggtgtaaaga ttcaggcttc cgtggttctt gatgccactg ggttttcccg atgcttggtt
     2941 cagtatgaca aaccttacaa ccctgggtac caagtagctt acgggattgt agctgaagtt
     3001 gatggtcacc cattcgatgt agacaaaatg gtgttcatgg attggagaga caaacatctg
     3061 gactcatatc ctgagctgaa agaacggaac agcaagatcc caacgttctt gtacgctatg
     3121 ccattttctt ccaaccgaat atttcttgaa gaaacttctt tagttgctag acctggtctg
     3181 agaatggaag atatccaaga aagaatggct gctagactga aacatctggg gatcaatgtg
     3241 aagaggattg aggaagacga gcgttgtgtg atcccgatgg gcggtccttt accagtctta
     3301 cctcaacggg ttgtggggat tggtgggaca gcaggaatgg ttcatccttc aactggttac
     3361 atggttgcta ggactcttgc agctgcacca atagttgcaa atgccattgt gagatacctc
     3421 ggttcaccaa gtagtaatag cctgagagga gatcaactct ctgctgaggt ttggagagac
     3481 ttgtggccta tcgaacggcg tagacagagg gagttcttct gttttggaat ggatattctg
     3541 ctgaaactcg atttagacgc tactagaagg ttctttgatg cattctttga tctgcaacct
     3601 cattactggc acggattctt gtcttccagg ctgtttctcc cggaactgtt ggtcttcggg
     3661 ttgtcgctct tctcacacgc ttccaatacc tcaagattgg agatcatgac aaaggggact
     3721 gttcctcttg ctaagatgat caacaatttg gtacaagata gagactaagg accagaaact
     3781 tagacatata agtacatctg ttctttggtt cttgaccagt agtatatccg cattgcaagt
     3841 cgttggataa ttgtgtataa accacagatc ataacctgaa tccttgtgaa atcaaattgt
     3901 tactactagt tcattaaaat taatactttg tgctgcattg tgtttcacca actcttgtaa
     3961 atccaaaact agaggcaaaa tgtaataaga taaaagagtg gattttgaac acaaaaaaaa
     4021 aaaaaaaaaa aaaaagggcg gccgctctag aggatccaag cttatcgata ccgtcgacct
     4081 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     4141 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4201 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4261 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa
     4321 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4381 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4441 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4501 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4561 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4621 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4681 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4741 gggcgcgtca g
//
