LOCUS       sequence-83631-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for N-term eGFP fusion, frame C1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-GFP-C1 (w392-1)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    197..262
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(305..326)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     654..673
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
       61 acgggccaga gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      181 tgggtctaga tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt
      241 gagctcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
      301 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      361 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      421 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      481 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
      541 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      601 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      661 agttgccgtc gtccttgaag a
//
