LOCUS       sequence-83641-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS14
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     420..441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(547..568)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(808..827)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tataacatgg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc tcgccaccat ggtcgactca tcacgtcgta agtggaataa gacacacgca
      181 gtcagagcta taggtcggct gagctcactc gagaacgtct atatcaaggc cgacaagcag
      241 aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
      301 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      361 aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
      421 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      481 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
      541 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      601 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      661 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      721 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      781 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      841 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      901 gacggcaaca tcctggggca caagctggag tacaacacgc gtgaccaact gactgaagag
      961 cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacaata
     1021 acaaccaagg agctggggac ggtgatgcgg tctctggg
//
