LOCUS       sequence-83644-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3-zeta (zeta) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez
            MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY
  TITLE     Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates 
            neurodegeneration in spinocerebellar ataxia type 1.
  JOURNAL   Cell. 2003 May 16;113(4):457-68.
  PUBMED    12757707
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2003-05-16"; volume: "113(4)"; pages: "457-68"
COMMENT     Sequence Label: pIRES-HA-14-3-3 ϵ/myc-14-3-3-zeta
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(52..78)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             896..922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aggctagagt acttaatacg actcactata ggctagcctc gagaattctc aagcataatc
       61 tggaacatca tatggatact gattttcgtc ttccacccgt cgcgcagacc ctgccggagc
      121 cgctgccgct atggatgatc gagaggatct ggtgtaccag gcgaagctgg ccgagcaggc
      181 tgagcgatac gacgaaatgg tggagtcaat gaagaaagta gcagggatgg atgtggagct
      241 gacagttgaa gaaagaaacc tcctatctgt tgcatataag aatgtgattg gagctagaag
      301 agcctcctgg agaataatca gcagcattga acagaaagaa gaaaacaagg gaggagaaga
      361 caagctaaaa atgattcggg aatatcggca aatggttgag actgagctaa agttaatctg
      421 ttgtgacatt ctggatgtac tggacaaaca cctcattcca gcagctaaca ctggcgagtc
      481 caaggttttc tattataaaa tgaaagggga ctaccacagg tatctggcag aatttgccac
      541 aggaaacgac aggaaggagg ctgcggagaa cagcctagtg gcttataaag ctgctagtga
      601 tattgcaatg acagaacttc caccaacgca tcctattcgc ttaggtcttg ctctcaattt
      661 ttccgtattc tactacgaaa ttcttaattc ccctgaccgt gcctgcaggt tggcaaaagc
      721 agcttttgat gatgcaattg cagaactgga tacgctgagt gaagaaagct ataaggactc
      781 tacacttatc atgcagttgt tacgtgataa tctgacacta tggacttcag acatgcaggg
      841 tgacggtgaa gagcagaata aagaagcgct gcaggacgtg gaagacgaaa atcagtatcc
      901 atatgatgtt ccagattatg cttgagaatt cacgcgtcga gcatgcatct agg
//
