LOCUS       sequence-83646-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS15
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     417..438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(544..565)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(805..824)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tataacatgg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc tcgccaccat ggtcgactca tcacgtcgta agtggaataa gacagcagtc
      181 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
      241 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
      301 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      361 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
      421 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      481 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      541 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
      601 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      661 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      721 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
      781 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      841 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      901 ggcaacatcc tggggcacaa gctggagtac aacacgcgtg accaactgac tgaagagcag
      961 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
     1021 accaagga
//
