LOCUS       sequence-83652-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible expression of link-NGFP negative control for in vivo 
            split GFP assembly assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, Hamilton AD, 
            Regan L
  TITLE     Detecting protein-protein interactions with a green fluorescent 
            protein fragment reassembly trap: scope and mechanism.
  JOURNAL   J Am Chem Soc. 2005 Jan 12;127(1):146-57.
  PUBMED    15631464
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2005-01-12"; volume: "127(1)"; pages: "146-57"
COMMENT     Sequence Label: pET11a-link-NGFP
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(206..234)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(736..754)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      750..797
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata tacatatggc tagccatcac caccatcacc atggcgcgag
      181 caaaggagaa gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      241 taacggccac aagttctctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      301 taccctgaag ttcatctgca ctactggcaa actgcctgtt ccatggccaa cactagtcac
      361 tactctgtgc tatggtgttc aatgcttttc aagatacccg gatcatatga aacggcatga
      421 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa aggaccatct tcttcaaaga
      481 tgacggcaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      541 aatcgagtta aaaggtattg acttcaagga agatggcaac attctgggac acaaattgga
      601 atacaactat aactcacaca acgttctcat catggcagac aaacaaggtg gctctggctc
      661 tggctcgagc aatcccggga attaaggatc cggctgctaa caaagcccga aaggaagctg
      721 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      781 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg
      841 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
      901 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
      961 taaactaccg cattaaagct
//
