LOCUS       sequence-83666-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Japanese Encephalitis Virus VLPs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Nour AM, Li Y, Wolenski J, Modis Y
  TITLE     Viral membrane fusion and nucleocapsid delivery into the cytoplasm 
            are distinct events in some flaviviruses.
  JOURNAL   PLoS Pathog. 2013;9(9):e1003585. doi: 10.1371/journal.ppat.1003585. 
            Epub 2013 Sep 5.
  PUBMED    24039574
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013"; volume: "9(9):e1003585. doi"; pages: " 
            10.1371/journal.ppat.1003585. Epub 2013 Sep 5"
COMMENT     Sequence Label: pcDNA3.1(+) prME (JEV) sdm2
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgcttgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc
      181 cgccaccatg ggaaatgaag gctcaatcat gtggctcgcg tgcttggcag ttgtcatagc
      241 ttatgcagga gcaatgaagt tgtcgaattt ccaggggaag cttttgatga ccatcaacaa
      301 cacggacatt gcagacgtta tcgtgattcc tacttcaaaa ggagagaaca gatgctgggt
      361 ccgggcaata gacgtcggct acatgtgtga ggacactatc acgtatgaat gtcccaagct
      421 taccatgggc aatgatccag aggatgtaga ttgctggtgt gacaaccaag aagtctacgt
      481 ccaatatgga cgatgcacgc ggactaggca ttccaagcga agcaggagat ccgtatcggt
      541 ccaaacacat ggggagagtt cactagtgaa taaaaaagag gcttggctgg attcaacgaa
      601 agccacacga tatctcatga aaactgagaa ctggatcata aggaatcctg gctacgctt
//
