LOCUS       sequence-83672-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta subunit for IKs channel in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Sanguinetti MC, Curran ME, Zou A, Shen J, Spector PS, Atkinson DL, 
            Keating MT
  TITLE     Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks)
            potassium channel.
  JOURNAL   Nature. 1996 Nov 7;384(6604):80-3.
  PUBMED    8900283
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1996-11-7"; volume: "384(6604)"; pages: "80-3"
COMMENT     Sequence Label: pSP64-hKCNE1
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(472..488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(472..488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(485..507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    496..512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(520..526,527..544,545..550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    565..586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(703..720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgtctaac accacagcgg tgacgccctt tctgaccaag ctgtggcagg agacagttca
       61 gcagggtggc aacatgtcgg gcctggcccg caggtccccc cgcagcggtg acggcaagct
      121 ggaggccctc tacgtcctca tggtactggg attcttcggc ttcttcaccc tgggcatcat
      181 gctgagctac atccgctcca agaagctgga gcactcgaac gacccattca acgtctacat
      241 cgagtccgat gcctggcaag agaaggacaa ggcctatgtc caggcccggg tcctggagag
      301 ctacaggtcg tgctatgtcg ttgaaaacca tctggccata gaacaaccca acacacacct
      361 tcctgagacg aagccttccc catgaacccc accactggct aaactggatc cccgggcgag
      421 ctcccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccgaa ttcgtaatca tgtcatagct
      481 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      541 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      601 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      661 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat a
//
